Discrimination of homologous chromosomes of maize with giemsa staining
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چکیده
منابع مشابه
The Giemsa-staining centromeres of Nigella damascena.
The centromere regions of each chromosome in the complement of Nigella damascena (2n equals 2x equals 12) stain differentially with Giemsa at interphase and throughout all the principal stages of mitosis and meiosis. Each centromere is seen to consist of a pair of sister half-centromeres which appear as 2 differentially stained dots. The appearance and behaviour of these dots indicates that the...
متن کاملImmunodetection of 5'-methylcytosine on Giemsa-stained chromosomes.
BioTechniques 527 In higher vertebrates, the major, if not the only, covalent modification of DNA is a methyl-group present at the C5 position of cytosine yielding 5′-methylcytosine (5′-MC). This modification occurs only in the context of CpG dinucleotides, short sequences that are significantly underrepresented in the genome but are found frequently concentrated within so called “CpG island” r...
متن کاملModified Giemsa staining for rapid diagnosis of malaria infection.
OBJECTIVES To develop and evaluate a rapid method for the diagnosis of malaria infection by microscopy of stained blood films. SUBJECTS AND METHODS Blood specimens were collected from randomly selected confirmed malaria cases (n = 75) and suspected malaria cases (n = 175). The microscopy was done on each set of blood films stained by modified and the standard Giemsa staining methods. RESULT...
متن کاملGiemsa staining for cysts and trophozoites of Pneumocystis carinii.
Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining w...
متن کاملThe Diagnosis of Secondary Syphilis by Gram and Giemsa Staining
A 21-year-old man presented with a painless ulcer on the penis, which had been present for 4 weeks, and diffuse, symmetrical erythematous maculopapules on the trunk, extremities, palms, and soles, which had been present for 1 week. Gram staining of bloody exudate from his chancre revealed very weakly Gram-negative spiral rods (Picture 1; × 1,000). We therefore performed Giemsa staining, which r...
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ژورنال
عنوان ژورنال: Heredity
سال: 1975
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.1975.107